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Influence of ammonium chloride feeding time and light intensity on the cultivation of Spirulina (Arthrospira) platensis by Raquel Pedrosa Bezerra; Marcelo Chuei Matsudo; Attilio Converti; Sunao Sato; João Carlos Monteiro de Carvalho is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Influence of ammonium chloride feeding time and light intensity on the cultivation of Spirulina (Arthrospira) platensis about?

## Abstract This study dealt with the influence of both the feeding time and light intensity on the fed‐batch culture of the cyanobacterium __Spirulina__ (__Arthrospira__) __platensis__ using ammonium chloride as a nitrogen source. For this purpose, a 2^2^ plus star central composite experimental design combined with response surface methodology was employed, and the maximum cell concentration (__X__~m~), the cell productivity (__P__~__X__~), and the yield of biomass on nitrogen (__Y__~__X__/__N__~) were selected as the response variables. The optimum values of __X__~m~ (1,833 mg L^−1^) and __Y__~__X__/__N__~ (5.9 g g^−1^) estimated by the model at light intensity of 13 klux and feeding time of 17.2 days were very close to those obtained experimentally under these conditions (__X__~m~ = 1,771 ± 41 mg L^−1^; __Y__~__X__/__N__~ = 5.7 ± 0.17 g g^−1^). The cell productivity was a decreasing function of the ammonium chloride feeding time and a quadratic function of the light intensity. The protein and lipid contents of dry biomass collected at the end of cultivations were shown to decrease with increasing light intensity. Biotechnol. Bioeng. 2008;100: 297–305. © 2007 Wiley Periodicals,

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Author
Raquel Pedrosa Bezerra; Marcelo Chuei Matsudo; Attilio Converti; Sunao Sato; João Carlos Monteiro de Carvalho
Publisher
John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
Published
2007
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)