Can I read PED/PEA-15 controls fibroblast motility and wound closure by ERK1/2-dependent mechanisms on EtoBox?

PED/PEA-15 controls fibroblast motility and wound closure by ERK1/2-dependent mechanisms by Roberta Buonomo; Ferdinando Giacco; Angela Vasaturo; Sergio Caserta; Stefano Guido; Valentina Pagliara; Corrado Garbi; Gelsomina Mansueto; Angela Cassese; Giuseppe Perruolo; Francesco Oriente; Claudia Miele; Francesco Beguinot; Pietro Formisano is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is PED/PEA-15 controls fibroblast motility and wound closure by ERK1/2-dependent mechanisms about?

Cell migration is dependent on the control of signaling events that play significant roles in creating contractile force and in contributing to wound closure. We evaluated wound closure in fibroblasts from mice overexpressing (TgPED) or lacking ped/pea-15 (KO), a gene overexpressed in patients with type 2 diabetes. Cultured skin fibroblasts isolated from TgPED mice showed a significant reduction in the ability to recolonize wounded area during scratch assay, compared to control fibroblasts. This difference was observed both in the absence and in the presence of mytomicin C, an inhibitor of mitosis. In time-lapse experiments, TgPED fibroblasts displayed about twofold lower velocity and diffusion coefficient, as compared to controls. These changes were accompanied by reduced spreading and decreased formation of stress fibers and focal adhesion plaques. At the molecular level, TgPED fibroblasts displayed decreased RhoA activation and increased abundance of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2). Inhibition of ERK1/2 activity by PD98059 restored RhoA activation, cytoskeleton organization and cell motility, and almost completely rescued wound closure of TgPED

Who reads PED/PEA-15 controls fibroblast motility and wound closure by ERK1/2-dependent mechanisms?

Author

Roberta Buonomo; Ferdinando Giacco; Angela Vasaturo; Sergio Caserta; Stefano Guido; Valentina Pagliara; Corrado Garbi; Gelsomina Mansueto; Angela Cassese; Giuseppe Perruolo; Francesco Oriente; Claudia Miele; Francesco Beguinot; Pietro Formisano

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2012

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)