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Recombinant glycodelin carrying the same type of glycan structures as contraceptive glycodelin-A can be produced in human kidney 293 cellsbut not in Chinese hamster ovary cells by Ingrid M. Van den Nieuwenhof; Hannu Koistinen; Richard L. Easton; Riitta Koistinen; Meerit Kämäräinen; Howard R. Morris; Irma van Die; Markku Seppälä; Anne Dell; Dirk H. Van den Eijnden is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We have produced human recombinant glycodelin in human kidney 293 cells and in Chinese hamster ovary (CHO) cells. Structural analyses by lectin immunoassays and fast atom bombardment mass spectrometry showed that recombinant human glycodelin produced in CHO cells contains only typical CHO‐type glycans and is devoid of any of the __N__,__N′‐__diacetyllactosediamine (lacdiNAc)‐based chains previously identified in glycodelin‐A (GdA). By contrast, human kidney 293 cells produced recombinant glycodelin with the same type of carbohydrate structures as GdA. The presence of a β1→4‐__N‐__acetylgalactosaminyltransferase functioning in the synthesis of lacdiNAc‐based glycans in human kidney 293 cells is concluded to be the cause of the occurrence of lacdiNAc‐based glycans on glycodelin produced in these cells. Furthermore, human kidney 293 cells were found to be particularly suited for the production of recombinant glycodelin when they were cultured in high glucose media. Lowering the glucose concentration and the addition of glucosamine resulted in higher relative amounts of oligomannosidic‐type glycans and complex glycans with truncated antennae. Human glycodelin is an attractive candidate

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Author

Ingrid M. Van den Nieuwenhof; Hannu Koistinen; Richard L. Easton; Riitta Koistinen; Meerit Kämäräinen; Howard R. Morris; Irma van Die; Markku Seppälä; Anne Dell; Dirk H. Van den Eijnden

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)