Can I read De Novo Design, Synthesis and Study of Albebetin, a Polypeptide with a Predetermined Three-dimensional Structure on EtoBox?
De Novo Design, Synthesis and Study of Albebetin, a Polypeptide with a Predetermined Three-dimensional Structure by A.N. Fedorov; D.A. Dolgikh; V.V. Chemeris; B.K. Chernov; A.V. Finkelstein; A.A. Schulga; Yu.B. Alakhov; M.P. Kirpichnikov; O.B. Ptitsyn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is De Novo Design, Synthesis and Study of Albebetin, a Polypeptide with a Predetermined Three-dimensional Structure about?
The de novo polypeptide named albebetin was designed to form the tertiary fold that has not yet been observed in natural proteins. The design was based on the molecular theory of protein structures. The gene coding for this polypeptide was chemically synthesized. For the initial characterization of a protein structure, a new approach has been developed that uses only nanogram amounts of a polypeptide without its previous purification. This approach includes the biosynthesis of radiolabeled protein in a cell-free translation system with subsequent analysis of its compactness and structure by size-exclusion chromatography, urea-gradient electrophoresis and limited proteolysis. According to all tests used, albebetin has a compact stable structure.
Who reads De Novo Design, Synthesis and Study of Albebetin, a Polypeptide with a Predetermined Three-dimensional Structure?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- A.N. Fedorov; D.A. Dolgikh; V.V. Chemeris; B.K. Chernov; A.V. Finkelstein; A.A. Schulga; Yu.B. Alakhov; M.P. Kirpichnikov; O.B. Ptitsyn
- Publisher
- Elsevier BV
- Published
- 1992
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)