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A DNA-Based MRI Contrast Agent for Quantitative pH Measurement by Hyewon Seo; Kristine Y. Ma; Erin E. Tuttle; Isen Andrew C. Calderon; Alissa D. Buskermolen; Chris A. Flask; Heather A. Clark is a Engineering article available to read on EtoBox.

What is A DNA-Based MRI Contrast Agent for Quantitative pH Measurement about?

Extracellular pH is important in clinical measurements due to its correlation to cell metabolism and disease progression. In MRI, T 1 /T 2 ratiometric analysis and other methods have been previously applied to quantify pH using conventional pulse sequences. However, for nanoparticle-based approaches, heterogeneity in size and surface functionalization tends toward qualitative rather than quantitative results. To address this limitation, we developed a novel DNA-based MRI contrast agent, pH-DMRCA, which utilizes a highly programmable and reproducible nanostructure. The pH-DMRCA is a dendritic DNA scaffold that is functionalized with a pH-responsive MRI-sensitive construct, Gd(NP-DO3A), at the end of each DNA arm. We first evaluated the r 1 and r 2 response of our pH-DMRCA over a range of pH values (pH = 5-9) to establish a relaxometric model of pH. These MRIbased assessments of pH were validated in a separate set of samples using a pH electrode (n = 18) and resulted in a good linear correlation (R 2 = 0.99, slope = 0.98, intercept = 0). A Bland-Altman analysis of the results also showed reasonable agreement between the calculated pH and measured pH. Moreover, these pH comparisons we

Who reads A DNA-Based MRI Contrast Agent for Quantitative pH Measurement?

Author

Hyewon Seo; Kristine Y. Ma; Erin E. Tuttle; Isen Andrew C. Calderon; Alissa D. Buskermolen; Chris A. Flask; Heather A. Clark

Publisher

American Chemical Society (ACS)

Published

2021

Language

EN

Field

Engineering (Physical Sciences)