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Bacterial Degradation of 3-Chloroacrylic Acid and the Characterization of Cis- and Trans-specific Dehalogenases by Johan E. T. van Hylckama Vlieg; Dick B. Janssen is a Environmental Science article available to read on EtoBox.

What is Bacterial Degradation of 3-Chloroacrylic Acid and the Characterization of Cis- and Trans-specific Dehalogenases about?

A coryneform bacterium that is able to utilize cisand trans-3-chloroacrylic acid as sole carbon source for growth was isolated from freshwater sediment. The organism was found to produce two inducible dehalogenases, one specific for the cisand the other for trans-3-chloroacrylic acid. Both dehalogenases were purified to homogeneity from cells induced for dehalogenase synthesis with 3-chlorocrotonic acid. The enzymes produced muconic acid semialdehyde (3-oxopropionic acid) from their respective 3-chloroacrylic acid substrate. No other substrates were found. The cis-3-chloroacrylic acid dehalogenase consisted of two polypeptide chains of a molecular weight 16.2 kDa. Trans-3-chloroacrylic acid dehalogenase was a protein with subunits of 7.4 and 8.7 kDa. The subunit and amino acid compositions and the N-terminal amino acid sequences of the enzymes indicate that they are not closely related.

Who reads Bacterial Degradation of 3-Chloroacrylic Acid and the Characterization of Cis- and Trans-specific Dehalogenases?

Author

Johan E. T. van Hylckama Vlieg; Dick B. Janssen

Publisher

Springer Netherlands; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0923-9820)

Published

1991

Language

EN

Field

Environmental Science (Physical Sciences)