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Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals by Michael Z. Lin; Michael R. McKeown; Ho-Leung Ng; Todd A. Aguilera; Nathan C. Shaner; Robert E. Campbell; Stephen R. Adams; Larry A. Gross; Wendy Ma; Tom Alber; Roger Y. Tsien is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals about?

Fluorescent proteins have become valuable tools for biomedical research as protein tags, reporters of gene expression, biosensor components, and cell lineage tracers. However, applications of fluorescent proteins for deep tissue imaging in whole mammals have been constrained by the opacity of tissues to excitation light below 600 nm, because of absorbance by hemoglobin. Fluorescent proteins that excite efficiently in the "optical window" above 600 nm are therefore highly desirable. We report here the evolution of far-red fluorescent proteins with peak excitation at 600 nm or above. The brightest one of these, Neptune, performs well in imaging deep tissues in living mice. The crystal structure of Neptune reveals a novel mechanism for red-shifting involving the acquisition of a new hydrogen bond with the acylimine region of the chromophore.

Who reads Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals?

Author

Michael Z. Lin; Michael R. McKeown; Ho-Leung Ng; Todd A. Aguilera; Nathan C. Shaner; Robert E. Campbell; Stephen R. Adams; Larry A. Gross; Wendy Ma; Tom Alber; Roger Y. Tsien

Publisher

Elsevier Science; Elsevier ; Cell Press; Elsevier BV (ISSN 1074-5521)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)