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Localization of cytosolic aldehyde dehydrogenase in the developing chick retina: In situ hybridization and immunohistochemical analyses by Roseline Godbout; Mary Packer; Sibrand Poppema; Laith Dabbagh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Localization of cytosolic aldehyde dehydrogenase in the developing chick retina: In situ hybridization and immunohistochemical analyses about?

Cytosolic aldehyde dehydrogenase (ALDH) mRNA is present at high levels in the undifferentiated chick retina. Tissue maturation is accompanied by a 20-25x decrease in transcript levels. To determine the spatial and temporal distribution pattern of the ALDH transcript and its encoded protein in the developing retina, in situ hybridization and immunohistochemical analyses were carried out using chick embryos at different stages of development. The ALDH transcript and protein were detected at the earliest stage tested, in the inner layer of the optic cup of stage 14 (day 2) embryos. Both the ALDH transcript and protein were found in the dorsal retina of chick embryos from stage 18 (day 3) to day 16 of incubation. Accumulation of the ALDH protein in the neurites of ganglion cells could readily be detected at early developmental stages. Staining of this ganglion fiber layer was strong in the dorsal retina and could be followed up to and into the optic nerve. By day 11, ALDH mRNA was located primarily in the ciliary margin and in the inner nuclear layer of the dorsal retina. In addition to these areas, the ALDH protein was also found in the inner plexiform and optic nerve fiber layers. Th

Who reads Localization of cytosolic aldehyde dehydrogenase in the developing chick retina: In situ hybridization and immunohistochemical analyses?

Author

Roseline Godbout; Mary Packer; Sibrand Poppema; Laith Dabbagh

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)