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Can I read Electroporation-based Methods for in Vivo , Whole Mount and Primary Culture Analysis of Zebrafish Brain Development on EtoBox?
Electroporation-based Methods for in Vivo , Whole Mount and Primary Culture Analysis of Zebrafish Brain Development by Michael Hendricks; Suresh Jesuthasan is a Neuroscience article available to read on EtoBox.
What is Electroporation-based Methods for in Vivo , Whole Mount and Primary Culture Analysis of Zebrafish Brain Development about?
## Background Electroporation is a technique for the introduction of nucleic acids and other macromolecules into cells. In chick embryos it has been a particularly powerful technique for the spatial and temporal control of gene expression in developmental studies. Electroporation methods have also been reported for __Xenopus__, zebrafish, and mouse. ## Results We present a new protocol for zebrafish brain electroporation. Using a simple set-up with fixed spaced electrodes and microinjection equipment, it is possible to electroporate 50 to 100 embryos in 1 hour with no lethality and consistently high levels of transgene expression in numerous cells. Transfected cells in the zebrafish brain are amenable to __in vivo__ time lapse imaging. Explants containing transfected neurons can be cultured for __in vitro__ analysis. We also present a simple enzymatic method to isolate whole brains from fixed zebrafish for immunocytochemistry. ## Conclusion Building on previously described methods, we have optimized several parameters to allow for highly efficient unilateral or bilateral transgenesis of a large number of cells in the zebrafish brain. This method is simple and provides consistently
Who reads Electroporation-based Methods for in Vivo , Whole Mount and Primary Culture Analysis of Zebrafish Brain Development?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Michael Hendricks; Suresh Jesuthasan
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2006-; Springer Science and Business Media LLC (ISSN 1749-8104)
- Published
- 2007
- Language
- EN
- Field
- Neuroscience (Life Sciences)