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A Procedure for the Analysis of Acid-soluble Phosphorus Compounds and Related Substances in Muscle and Other Tissues by T. Wood is a Chemistry article available to read on EtoBox.

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In 1956, THRELFALL 1 published a procedure enabling him to separate and estimate quantitatively IO different phosphorus compounds present in an acid extract of rat muscle. The chromatographic methods used to separate these substances on paper were described only briefly. The object of the present work has been to enlarge and extend the methods of THRELFALL 1 and other workers so as to develop a simple but comprehensive procedure for investigating phosphorus compounds in aqueous tissue extracts. Procedures for the water-soluble non-phosphorylated compounds have already been described by the author 2-4. ## MATERIALS AND EQUIPMENT Chromatography was carried out with the "Universal Chromatography Outfit" supplied by Aimer Products Ltd.\*. The apparatus was adapted by the author to eliminate all metallic parts. Trays were constructed of 1/4 in. polythene sheet welded together at the edges; the frames consisted of 1/4 in. polythene sheet joined by four 8 mm diameter glass rods, and the spacers were of i 1/4 in. lengths of polythene tubing, 3/8 in. internal diameter. All chromatograms were of the ascending type on IO in. square sheets of Whatman No. 541 paper. Norit SX 30 Special was a gi

Who reads A Procedure for the Analysis of Acid-soluble Phosphorus Compounds and Related Substances in Muscle and Other Tissues?

Author

T. Wood

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1961

Language

EN

Field

Chemistry (Physical Sciences)