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This study investigates the enhancement of DNA polymerases from family A through metagenomic analysis, focusing on an insertion sequence in the finger subdomain that improves DNA binding and primer extension abilities. The modified Taq polymerases, derived from Thermus aquaticus, exhibit superior PCR performance due to this insertion. The findings suggest that these engineered enzymes could significantly advance PCR efficiency and effectiveness.

Author

Dat Le Mai Tan

Language

EN