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Can I read High-performance liquid chromatography of transfer RNAs on EtoBox?
High-performance liquid chromatography of transfer RNAs by Ram P. Singhal is a Chemistry article available to read on EtoBox.
What is High-performance liquid chromatography of transfer RNAs about?
A survey of recent advances in high-performance liquid chromatography (HPLC) of tRNA is presented here. The polystyrene and reversed-phase anion exchangers are discussed for their ability to resolve tRNAs without loss of the aminoacyl-tRNA bond. The HPLC of a tRNA of choice, based on the affinity principle, is studied. Both chemical (boronate) and biological (plant lectins) affinity groups for the tRNA interaction are described. A comprehensive scheme is presented for the separation of four mammalian tRNAs. Scope of future research in this area is also discussed. \* One A,,, unit is the amount of substance in 1 ml of 50 mM sodium acetate buffer with 10 mM MgCI, (pH 4.5) which gives an absorbance of 1.0 when measured at 260 nm with a light path of 10 mm.
Who reads High-performance liquid chromatography of transfer RNAs?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Ram P. Singhal
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1983
- Language
- EN
- Field
- Chemistry (Physical Sciences)