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Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405 by Thomas Rydzak; David B. Levin; Nazim Cicek; Richard Sparling is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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End-product synthesis and enzyme activities involved in pyruvate catabolism, H 2 synthesis, and ethanol production in mid-log (OD 600 ∼ 0.25), early stationary (OD 600 ∼ 0.5), and stationary phase (OD 600 ∼ 0.7) cell extracts were determined in Clostridium thermocellum ATCC 27405 grown in batch cultures on cellobiose. Carbon dioxide, hydrogen, ethanol, acetate and formate were major endproducts and their production paralleled growth and cellobiose consumption. Lactate dehydrogenase, pyruvate:formate lyase, pyruvate:ferredoxin oxidoreductase, methyl viologen-dependant hydrogenase, ferredoxin-dependant hydrogenase, NADH-dependant hydrogenase, NADPH-dependant hydrogenase, NADH-dependant acetaldehyde dehydrogenase, NADH-dependant alcohol dehydogenase, and NADPHdependant alcohol dehydrogenase activities were detected in all extracts, while pyruate dehydrogenase and formate dehydrogenase activities were not detected. All hydrogenase activities decreased (2-12fold) as growth progressed from early exponential to stationary phase. Alcohol dehydrogenase activities fluctuated only marginally (<45%), while lactate dehydrogenase, pyruvate:formate lyase, and pyruvate:ferredoxin oxidoreductase re

Who reads Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405?

Author

Thomas Rydzak; David B. Levin; Nazim Cicek; Richard Sparling

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-1656)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)