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Sensitive and specific serodiagnosis of tegumentary leishmaniasis using a new chimeric protein based on specific B-cell epitopes of Leishmania antigenic proteins by Nathalia C. Galvani; Amanda S. Machado; Daniela P. Lage; Vívian T. Martins; Daysiane de Oliveira; Camila S. Freitas; Danniele L. Vale; Bruna B. Fernandes; João A. Oliveira-da-Silva; Thiago A.R. Reis; Thaís T.O. Santos; Fernanda F. Ramos; Raquel S. Bandeira; Fernanda Ludolf; Grasiele S.V. Tavares; Nathalia S. Guimarães; Unaí Tupinambás; Miguel A. Chávez-Fumagalli; Maria V. Humbert; Denise U. Gonçalves; Myron Christodoulides; Ricardo A. Machado-de-Ávila; Eduardo A.F. Coelho is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Sensitive and specific serodiagnosis of tegumentary leishmaniasis using a new chimeric protein based on specific B-cell epitopes of Leishmania antigenic proteins about?

Serological tests used for the diagnosis of tegumentary leishmaniasis (TL) presents problems, mainly related to their variable sensitivity and/or specificity, which can be caused by low levels of antileishmanial antibodies or by presence of cross-reactive diseases, respectively. In this context, the search for new antigenic candidates presenting higher sensitivity and specificity is urgently required. In the present study, the amino acid sequences of the LiHyT, LiHyD, LiHyV, and LiHyP proteins, which were previously showed to be antigenic in the visceral leishmaniasis (VL), were evaluated and eight B-cell epitopes were predicted and used for construction of gene codifying a chimeric protein called ChimLeish. The protein was expressed, purified and evaluated as a recombinant antigen in ELISA (Enzyme-Linked Immunosorbent Assay) for the diagnosis of TL. The own B cell epitopes used to construct the chimera were synthetized and also evaluated as antigens, as well as a soluble Leishmania braziliensis antigenic extract (SLA). Results showed that ChimLeish presented 100% sensitivity and specificity to diagnose TL, while synthetic peptides showed sensitivity varying from 9.1% to 90.9%, whi

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Author

Nathalia C. Galvani; Amanda S. Machado; Daniela P. Lage; Vívian T. Martins; Daysiane de Oliveira; Camila S. Freitas; Danniele L. Vale; Bruna B. Fernandes; João A. Oliveira-da-Silva; Thiago A.R. Reis; Thaís T.O. Santos; Fernanda F. Ramos; Raquel S. Bandeira; Fernanda Ludolf; Grasiele S.V. Tavares; Nathalia S. Guimarães; Unaí Tupinambás; Miguel A. Chávez-Fumagalli; Maria V. Humbert; Denise U. Gonçalves; Myron Christodoulides; Ricardo A. Machado-de-Ávila; Eduardo A.F. Coelho

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)