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Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins by María Rosa Insenser; María Luisa Hernáez; César Nombela; María Molina; Gloria Molero; Concha Gil is a Chemistry article available to read on EtoBox.

What is Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins about?

The study of Saccharomyces cerevisiae cell surface proteins was performed because of their important role in cell wall biogenesis and in the physiology of the yeast. Two different proteomic approaches were carried out. First, proteins loosely associated or S-S linked to structural wall components were released by treatment of whole intact cells with dithiothreitol, separated by 2D-PAGE and identified by mass spectrometry. Second, cell surface-exposed proteins (surfome) were digested with trypsin and DTT from whole intact cells, and analyzed by LC-MS/MS. Ninety-nine different proteins were identified: 67 with DTT treatment and 52 with DTT and trypsin digestion. These proteins were classified in different cellular processes: control of cell wall organization, cell rescue, defence, and virulence, protein fate, protein synthesis and metabolism. Most of the proteins have already been reported as present on the cell surface showing that the yeast cell surface is composed not only by typical but also by atypical cell wall proteins. "Bona fide" cell wall proteins were identified by both protocols but a higher number with the non-gel strategy. However, only 20% of the proteins identified we

Who reads Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins?

Author

María Rosa Insenser; María Luisa Hernáez; César Nombela; María Molina; Gloria Molero; Concha Gil

Publisher

Elsevier ; Elsevier BV (ISSN 1874-3919)

Published

2010

Language

EN

Field

Chemistry (Physical Sciences)