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Increased Expression of Heat-Shock Protein 47 is Associated with Overproduction of Type I Procollagen in Systemic Sclerosis Skin Fibroblasts by Kuroda, Kei; Tsukifuji, Reiko; Shinkai, Hiroshi is a Medicine article available to read on EtoBox.

What is Increased Expression of Heat-Shock Protein 47 is Associated with Overproduction of Type I Procollagen in Systemic Sclerosis Skin Fibroblasts about?

Heat-shock protein 47 (HSP47) is a collagen-binding stress protein that is thought to act as a collagen-specific molecular chaperon during the biosynthesis and secretion of procollagen. In this study we examined the expression of HSP47 mRNA and protein in systemic sclerosis (SSc) skin fibroblasts. HSP47 mRNA and protein levels were significantly higher in fibroblast cultures from SSc patient-involved skin samples than in fibroblasts from normal skin from healthy individuals, as assessed by northern blot and immunoblot analyses, respectively. SSc cultured fibroblasts with increased levels of HSP47 mRNA and protein showed high expression of type I procollagen. By in situ hybridization, SSc skin had a higher number of fibroblasts with high HSP47 and procollagen alpha1(I) mRNA levels than normal skin, and the distribution of HSP47 mRNA was similar to that of procollagen alpha1(I) mRNA. We also investigated the effects of cytokines on the expression of HSP47 in normal cultured fibroblasts. Transforming growth factor-beta1 and interleukin-4 increased HSP47 mRNA and protein levels, whereas interferon-gamma reduced HSP47 expression. The same pattern of cytokine-regulated expression was obs

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Author

Kuroda, Kei; Tsukifuji, Reiko; Shinkai, Hiroshi

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; Springer Science and Business Media LLC; Wiley (ISSN 0022-202X)

Published

1998

Language

EN

Field

Medicine (Health Sciences)