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An immunochemical investigation of 2′:3′-cyclic nucleotide 3′ -phosphodiesterase (CNP) in bovine cerebrum and human oligodendroglioma by H.J. Sheedlo; F. Yaghmai; L. Wolfe III; Dr. T.J. Sprinkle is a Neuroscience article available to read on EtoBox.

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Bovine cerebrum, including the corpus callosum, and a human oligodendroglioma were investigated by an immunohistochemical technique to determine the distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP). Also, three human oligodendrogliomas (ODG) were characterized by an immunoblot procedure to identify a protein(s) with cross-reacting determinants to CNP and assayed for CNP activity. CNP was localized to oligodendrocytes in the corpus callosum and subcortical white matter and gray matter. Also, nerve fibers appeared to be stained. Further, cells of the human oligodendroglioma were immunostained which were similar in morphology to those cells stained in the bovine cerebrum; however, fewer than 5 % of the oligodendroglioma cells were immunostained. Immunoblotting revealed two separate and distinct bands for the three oligodendrogliomas, showing cross-reactivity to bovine CNP antisera at about 53,000 and 46,000 daltons. Specific CNP activity of the three human oligodendrogliomas ranged from 0.4 to 1.6 pmole of 2':3'-cAMP hydrolyzedlminimg protein.

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Author

H.J. Sheedlo; F. Yaghmai; L. Wolfe III; Dr. T.J. Sprinkle

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1984

Language

EN

Field

Neuroscience (Life Sciences)