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Can I read Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 on EtoBox?
Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 by Yizhao Zhou; Zhen Jia; Jing Wang; Shu Huang; Shu Yang; Sheng Xiao; Duo Xia; Yi Zhou is a Medicine article available to read on EtoBox.
What is Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 about?
Osteoarthritis (OA) is associated with ferroptosis, a newly discovered form of programmed cell death associated with lipid peroxidation. Curcumin, the main monomer component in turmeric rhizomes, possesses antioxidant and anti-ferroptosis properties, but its effect on ferroptosis in chondrocytes of OA is unknown. This study aimed to investigate the protective effect and potential mechanism of curcumin on chondrocytes induced by erastin, a ferroptosis inducer. CCK-8 assays were used to assess cell viability in mouse primary chondrocytes treated with 3.33 μM erastin alone or in combination with different doses of curcumin. Various parameters were detected, including LDH, SOD, GSH-PX, MDA, ROS and Fe^2+^ contents. The ferroptosis-related proteins, such as SLC7A11, GPX4, TFR1, ACSL4, and FTH1, were examined using immunofluorescence and western blotting. Nrf2 was knocked down using siRNA to explore the molecular mechanism through which curcumin protects chondrocytes from erastin-induced ferroptosis. In a mouse model of knee ferroptosis induced by intracavity injection of 10 μL erastin (5 mg/mL), HE staining, Safranin O-Fast Green staining, and immunohistochemistry were employed to evalu
Who reads Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Yizhao Zhou; Zhen Jia; Jing Wang; Shu Huang; Shu Yang; Sheng Xiao; Duo Xia; Yi Zhou
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Medicine (Physical Sciences)