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Identification of three cultivated varieties of Scutellaria baicalensis using the complete chloroplast genome as a super-barcode by Yuan Jiang; Chenghao Zhu; Shangtao Wang; Fusheng Wang; Zhirong Sun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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__Scutellaria baicalensis__ has been one of the most commonly used traditional Chinese medicinal plants in China for more than 2000 years. The three new varieties cultivated could not be distinguished by morphology before flowering. It will hinder the promotion of later varieties. Chloroplast DNA has been widely used in species identification. Moreover, previous studies have shown that complete chloroplast genome sequences have been suggested as super barcodes for identifying plants. Therefore, we sequenced and annotated the complete chloroplast genomes of three cultivated varieties. The chloroplast genomes of SBW, SBR, and SBP were 151,702 bp, 151,799 bp, and 151,876 bp, which contained 85 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The analysis of the repeat sequences, codon usage, and comparison of chloroplast genomes shared a high degree of conservation. However, the sliding window results show significant differences among the three cultivated varieties in __mat__K-__rps__16 and __pet__A-__psb__J. And we found that the __mat__K-__rps__16 sequence can be used as a barcode for the identification of three varieties. In addition, the complete chloroplast genome contains

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Author

Yuan Jiang; Chenghao Zhu; Shangtao Wang; Fusheng Wang; Zhirong Sun

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)