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Determination of Biologically Active Low-molecular-mass Thiols in Human Blood by A.R Ivanov; I.V Nazimov; L.A Baratova is a Chemistry article available to read on EtoBox.
What is Determination of Biologically Active Low-molecular-mass Thiols in Human Blood about?
A new high-performance capillary electrophoresis assay for aminothiols in human blood, including homocysteine, a marker of several human metabolism disorders, has been developed. Sample preparation involves conversion of disulfides to free thiols with triphenylphosphine, precipitation of proteins with sulfosalicylic acid, and conjugation of the thiols with monobromobimane. Derivatized thiols were separated in a sodium phosphate buffer using a fused-silica capillary (65 cm x 50 microm I.D.) at 30 degrees C. With the electric field of 250 V cm(-1), separation of homocysteine, glutathione and cysteine occurred at less than 10 min. Detection at 250 or 234 nm was used to confirm the monobimane-thiols peaks. The detection limit was approximately 5 nmol/ml for all labeled aminothiols. The proposed method for these compounds' analysis included simple sample preparation, high selectivity, good linearity (r2>0.999), high reproducibility (within-run precision for derivatized aminothiol peaks area RSD<5% for three times consequently injected sample); high reliability and the small volumes required for analysis made it suitable for clinical studies.
Who reads Determination of Biologically Active Low-molecular-mass Thiols in Human Blood?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- A.R Ivanov; I.V Nazimov; L.A Baratova
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2000
- Language
- EN
- Field
- Chemistry (Physical Sciences)