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Ultrasensitive nanohybrid electrochemical sensor to detect LipL32 gene of Leptospira interrogans by Rupak Nagraik; Sunil Sethi; Avinash Sharma; Deepak Kumar; Dinesh Kumar; Avvaru Praveen Kumar is a Chemistry article available to read on EtoBox.

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We report an ultrasensitive nanohybrid electrochemical DNA sensor developed to detect the LipL32 gene of Leptospira interrogans that causes leptospirosis. A carboxylated multiwalled carbon nanotubes electrode with gold nanoparticles (c-MWC-NTs/nano-Au) was modified with polyamidoamine (PAMAM) and graphene quantum dots using cysteine (Cys) as a linker. The c-MWCNTs/nano-Au electrode was covalently bonded to polyamidoamine (PAMAM) via Cys through Au-SH bonding. The amino group of PAMAM was linked to carboxyl moiety of graphene quantum dots (GQD) to form a c-MWCNTs/ nano-Au/Cys/PAMAM/GQD nanohybrid electrode. A 5'-amino (NH 2 ) labeled single-stranded DNA (ssDNA) probe based on the LipL32 gene was immobilized on the surface of a c-MWCNTs/nano-Au/Cys/PAMAM/GQD nanohybrid electrode. The different dilutions of single-stranded genomic DNA (ssG-DNA) of L. interrogans were treated on immobilized DNA probe to allow the hybridization. The hybridization events are measured using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) using redox indicators of 1 mM methylene blue and potassium ferricyanide. Field emission scanning electron

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Author

Rupak Nagraik; Sunil Sethi; Avinash Sharma; Deepak Kumar; Dinesh Kumar; Avvaru Praveen Kumar

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Chemistry (Physical Sciences)