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Active uptake of CO2 during photosynthesis in the green alga Eremosphaera viridis is mediated by a CO2-ATPase by Caterina Rotatore; Roger R. Lew; Brian Colman is a Agricultural and Biological Sciences article available to read on EtoBox.

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Mass spectrometry was used to investigate the uptake of CO2 in Eremosphaera viridis DeBary. Upon illumination, cells preincubated at pH 7.5 with 100 ~tM dissolved inorganic carbon (DIC) rapidly depleted almost all the free CO2 from the medium. Rapid equilibrium between HCO~ and CO2 occurred upon addition of bovine carbonic anhydrase (CA) to the medium, showing that CO2 depletion resulted from a selective uptake of CO2 rather than an uptake of all inorganic carbon species. Glycolaldehyde (10 mM) completely inhibited CO2 fixation but had little effect on CO2 transport. Transfer of glycolaldehyde-treated cells to the dark caused a rapid effiux of COz from the unfixed intracellular DIC pool which was found to be at least three-to sixfold higher in concentration than that of the external medium. These results indicate that E. viridis actively transports COz against a concentration gradient. No external CA was detected in these cells either by potentiometric or mass-spectrometric assay. In the absence of external CA, the rate of photosynthetic 02 evolution in the pH range 7.5 to 8.0 did not exceed the calculated rate of CO2 supply, indicating a limited capacity for HCOS uptake in these c

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Author

Caterina Rotatore; Roger R. Lew; Brian Colman

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1992

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)