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Purinergic Regulation of Acid/base Transport in Human and Rat Biliary Epithelial Cell Lines by Ákos Zsembery; Carlo Spirlì; Anna Granato; Nicholas F. LaRusso; Lajos Okolicsanyi; Gaetano Crepaldi; Mario Strazzabosco is a Medicine article available to read on EtoBox.

What is Purinergic Regulation of Acid/base Transport in Human and Rat Biliary Epithelial Cell Lines about?

Biliary epithelial cells (cholangiocytes) are responsible for rapid regulation of bile volume and alkalinity. Secretin and other hormones raising intracellular cyclic adenosine monophosphate (cAMP) concentrations promote biliary HCO3 secretion by stimulating apical Cl- channels and Cl-/HCO3- exchange (AE2). Cholangiocyte ion transport may also be stimulated by locally acting mediators; for example, adenosine 5'-triphosphate (ATP), a secretagogue that can be released into the bile by hepatocytes and cholangiocytes, activates Cl- conductances and Na+/H+ exchange (NHE) in cholangiocyte cell lines. To further explore the role of extracellular ATP in the paracrine regulation of carrier mechanisms regulating cholangiocyte H+/HCO3- secretion, we investigated the effects of nucleotides on intracellular pH regulation (measured by microfluorimetry with 2'7'-bis(2-carboxyethyl)-5,6,carboxyfluorescein [BCECF]) in human (MZ-ChA-1) and rat (NRC-1) cholangiocyte cell lines. In MZ-ChA-1 cells, 10 mol/L ATP, uridine 5'-triphosphate (UTP), and ATPgammas significantly increased NHE activity. The pharmacological profile of agonists was consistent with that anticipated for receptors of the P2Y2 class.

Who reads Purinergic Regulation of Acid/base Transport in Human and Rat Biliary Epithelial Cell Lines?

Author

Ákos Zsembery; Carlo Spirlì; Anna Granato; Nicholas F. LaRusso; Lajos Okolicsanyi; Gaetano Crepaldi; Mario Strazzabosco

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1998

Language

EN

Field

Medicine (Health Sciences)