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Modification of native collagen with cell‐adhesive peptide to promote RPE cell attachment on Bruch's membrane by Rizaldi Sistiabudi; John Paderi; Alyssa Panitch; Albena Ivanisevic is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Current efforts to reverse loss of visual function due to Age‐related Macular Degeneration point to the restoration of the Retinal Pigment Epithelial (RPE) layer. Restoration of the RPE layer involves replacing lost RPE cells as well as addressing the degeneration of the underlying Bruch's membrane (BM). To advance the potential of using donor BM, we present a strategy to achieve specific and controllable modification of the inner collagenous layer (ICL) of the Bruch's membrane. In particular, interaction between a collagen binding peptide (CBP) sequence with exposed collagen fibers on the ICL surface is utilized to anchor bioactive molecules. Here, a cell‐adhesion sequence is added to the collagen binding sequence to promote attachment and survival of ARPE‐19. First, the binding specificity of the CBP sequence is verified with a fluorescent binding assay. Subsequently, the effect of modification using the peptide is studied qualitatively using confocal fluorescent imaging and quantitatively through a cell proliferation assay. Results of these experiments indicate that the peptide sequence binds specifically to collagen fibers. Additionally, modification using the pepti

Who reads Modification of native collagen with cell‐adhesive peptide to promote RPE cell attachment on Bruch's membrane?

Author

Rizaldi Sistiabudi; John Paderi; Alyssa Panitch; Albena Ivanisevic

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)