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Can I read Rational Design and Characterisation of a Linear Cell Penetrating Peptide for Non-viral Gene Delivery on EtoBox?
Rational Design and Characterisation of a Linear Cell Penetrating Peptide for Non-viral Gene Delivery by Emma M. McErlean; Monika Ziminska; Cian M. McCrudden; John W. McBride; Stephen P. Loughran; Grace Cole; Eoghan J. Mulholland; Vicky Kett; Niamh E. Buckley; Tracy Robson; Nicholas J. Dunne; Helen O. McCarthy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Rational Design and Characterisation of a Linear Cell Penetrating Peptide for Non-viral Gene Delivery about?
The design of a non-viral gene delivery system that can release a functional nucleic acid at the intracellular destination site is an exciting but also challenging proposition. The ideal gene delivery vector must be non-toxic, non-immunogenic, overcome extra- and intra-cellular barriers, protect the nucleic acid cargo from degradation with stability over a range of temperatures. A new 15 amino acid linear peptide termed CHAT was designed in this study with the goal of delivering DNA with high efficiency into cells in vitro and tissues in vivo. Rational design involved incorporation of key amino acids including arginine for nucleic acid complexation and cellular uptake, tryptophan to enhance hydrophobic interaction with cell membranes, histidine to facilitate endosomal escape and cysteine for stability and controlled cargo release. Six linear peptides were synthesised with strategic sequences and amino acid substitutions. Data demonstrated that all six peptides complexed pDNA to produce cationic nanoparticles less than 200 nm in diameter, but not all peptides resulted in successful transfection; indicating the influence of peptide design for endosomal escape. Peptide 4, now termed C
Who reads Rational Design and Characterisation of a Linear Cell Penetrating Peptide for Non-viral Gene Delivery?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Emma M. McErlean; Monika Ziminska; Cian M. McCrudden; John W. McBride; Stephen P. Loughran; Grace Cole; Eoghan J. Mulholland; Vicky Kett; Niamh E. Buckley; Tracy Robson; Nicholas J. Dunne; Helen O. McCarthy
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-3659)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)