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Ultra-Multiplex CRISPR/Cas9 in Plants by fnm fnm is a document available to read on EtoBox.
What is Ultra-Multiplex CRISPR/Cas9 in Plants about?
This study presents a novel CRISPR/Cas9 plant ultra-multiplex genome editing system that allows for the targeting of over 40 genomic loci simultaneously. The system integrates Golden Gate cloning and Gateway recombination techniques, resulting in high co-editing efficiency demonstrated through the assembly of a rice knockout vector with 49 sgRNA expression cassettes. This advancement enhances the capabilities of synthetic biology and plant genetic engineering for improved crop traits.
- Author
- fnm fnm
- Language
- EN