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Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans by José Antonio Curiel; Ana Ruiz de la Bastida; Susana Langa; Ángela Peirotén; José María Landete is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans about?
## Abstract This study describes the characterization of the recombinant GH3 aryl-β-glucosidase “GluLm” from __Limosilactobacillus mucosae__ INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now. A high production of purified GluLm was achieved (14 mg L^−1^). GluLm exhibited optimal activity at broad ranges of pH (5.0–8.0) and temperature (25–60°C), as well as high affinity (__K__~__m__~ of 0.10 mmol L^−1^) and specific constant (86554.0 mmol L^−1^ s^−1^) against __p__-nitrophenyl-β-D-glucopyranoside. Similar to other GH3 β-glucosidases described in lactic acid bacteria, GluLm exhibited β-xylosidase, β-galactosidase, and β-fucosidase activities. However, this study has revealed for the first time that a GH3 β-glucosidase is capable to hydrolyze different families of glycosylated phenolics such as
Who reads Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- José Antonio Curiel; Ana Ruiz de la Bastida; Susana Langa; Ángela Peirotén; José María Landete
- Publisher
- Springer
- Published
- 2024
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)