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Can I read Motor Protein Independent Binding of Endocytic Carrier Vesicles to Microtubules in Vitro. on EtoBox?
Motor Protein Independent Binding of Endocytic Carrier Vesicles to Microtubules in Vitro. by J. Scheel; T.E. Kreis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Motor Protein Independent Binding of Endocytic Carrier Vesicles to Microtubules in Vitro. about?
We have established an in vitro assay to characterize the binding of endocytic carrier vesicles to microtubules. Magnetic beads coated with microtubules were used as an affinity matrix. A fraction from nocodazole-treated cells enriched in endocytic carrier vesicles, labeled with internalized horseradish peroxidase, was used in the binding experiments. Binding of the endocytic carrier vesicles to microtubules in vitro was cytosol-dependent. This activity of cytosolic factors was saturable, heat-sensitive, and insensitive to N-ethyl-maleimide. Binding was sensitive to GTP and ATP. Addition of neuronal microtubule-associated proteins completely abolished binding of the endocytic organelles to microtubules. This binding was independent of the cytosolic microtubule-based motor proteins kinesin and cytoplasmic dynein, since cytosol depleted of these proteins remained fully active. Microtubule-binding proteins from HeLa cells, however, stimulated the interaction of endocytic carrier vesicles with microtubules. Trypsinized vesicles could no longer bind to microtubules in the presence of cytosol. These results suggest that cytosolic microtubule-binding proteins, other than the known microtu
Who reads Motor Protein Independent Binding of Endocytic Carrier Vesicles to Microtubules in Vitro.?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- J. Scheel; T.E. Kreis
- Publisher
- Elsevier BV
- Published
- 1991
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)