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Effect of Phosphatidylcholine Chlorohydrins on Human Erythrocytes by Agnieszka Robaszkiewicz; Fiona H. Greig; Andrew R. Pitt; Corinne M. Spickett; Grzegorz Bartosz; Mirosław Soszyński is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Hypochlorite generated in vivo under pathological conditions is a known oxidant and chlorinating agent, able to react with proteins and lipids, which affects the stability of biological membranes. Reaction with unsaturated fatty acyl chains in glycerophospholipids such as phosphatidylcholine results in the formation of chlorohydrins. The aim of this study was to determine the effects of chlorohydrins formed by the reaction of hypochlorite with 1-stearoyl-2-oleoyl-, 1-stearoyl-2-linoleoyl-, and 1-stearoyl-2-arachidonylphosphatidylcholine on biophysical properties of bilayers and their effects on human erythrocytes. Using electrospray mass spectrometry we observed complete conversion of the lipids into chlorohydrins, which resulted in a decrease in the rotational correlation time and an increase in the order parameter of liposomes. Unilamellar chlorohydrin liposomes had a lower permeation coefficient for calcein than liposomes made of parent lipids. Flow cytometry demonstrated fast incorporation of uni and multilamellar chlorohydrin liposomes labeled with NBD-phosphatidylethanolamine into erythrocytes. This effect was accompanied by changes in erythrocyte shape (echinocyte formation)

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Author

Agnieszka Robaszkiewicz; Fiona H. Greig; Andrew R. Pitt; Corinne M. Spickett; Grzegorz Bartosz; Mirosław Soszyński

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-3084)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)