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Expression of sperm protein 17 (Sp17) in ovarian cancer by J. Michael Straughn Jr.; Denise R. Shaw; Antonio Guerrero; Snehal M. Bhoola; Alicia Racelis; Zhiqing Wang; Maurizio Chiriva-Internati; William E. Grizzle; Ronald D. Alvarez; Seah H. Lim; Theresa V. Strong is a Medicine article available to read on EtoBox.
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## Abstract Sperm protein 17 (Sp17) is an antigenic protein highly expressed in spermatozoa. Sp17 expression was demonstrated recently in multiple myeloma, suggesting that it may be a novel cancer‐testis antigen. Expression of Sp17 mRNA and protein was examined in human ovarian tumors. Sp17 mRNA was evaluated by reverse transcription‐polymerase chain reaction (RT‐PCR) and Northern blot analysis of RNA derived from epithelial ovarian tumors and normal tissues. RT‐PCR analysis detected Sp17 transcripts in 15 of 18 (83%) primary ovarian tumors. The transcript was not detected in RNA derived from normal uterus or cervix, whereas weak expression was noted in some normal ovarian tissue samples. Northern blot analysis showed no detectable Sp17 mRNA expression in normal tissues, including normal ovary, but showed Sp17 expression in 17 of 25 ovarian tumors (68%). To evaluate protein expression, mouse monoclonal antibodies were produced against recombinant Sp17 protein and used in Western blot and immunohistochemical analyses of normal reproductive tissue and primary ovarian tumor samples. Sp17 protein was detected by Western blot analysis in normal spermatozoa and in 8 of 19 ovarian tumor s
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- Author
- J. Michael Straughn Jr.; Denise R. Shaw; Antonio Guerrero; Snehal M. Bhoola; Alicia Racelis; Zhiqing Wang; Maurizio Chiriva-Internati; William E. Grizzle; Ronald D. Alvarez; Seah H. Lim; Theresa V. Strong
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)