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Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies by Anne-Sophie Bargnoux; Etienne Cavalier; Jean-Paul Cristol; Noémie Simon; Anne-Marie Dupuy; Valérie Garrigue; Stéphanie Badiou; Pierre Delanaye; Georges Mourad is a Medicine article available to read on EtoBox.
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## Abstract **Background:** The potential use of cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site human cystatin C immunoassay) has been developed. Analytical performance of this new assay was evaluated. Clinical relevance was determined by comparison with a reference method in a cohort of kidney transplant patients. **Methods:** First, the analytical performance of the Binding Site cystatin C kit was tested on SPA~PLUS~ ^®^ and Hitachi^®^ analyzers. Second, a comparison study was performed using SPA~PLUS~ ^®^ analyzer against two other cystatin C methods (the Siemens-PENIA method on BNII^®^ and the Dako-PETIA application on Olympus AU640^®^). Third, the glomerular filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based equations and compared to GFR measured by an isotopic method (^99m^Tc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy. **Results:** Total intra-assay and inter-assay coefficients of variation were below 5%. Values obtained with the SPA~PLUS~ ^®^ correlated with the Siemens-PE
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- Author
- Anne-Sophie Bargnoux; Etienne Cavalier; Jean-Paul Cristol; Noémie Simon; Anne-Marie Dupuy; Valérie Garrigue; Stéphanie Badiou; Pierre Delanaye; Georges Mourad
- Publisher
- Walter de Gruyter GmbH
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)