Can I read Advanced Technologies for Protein Complex Production and Characterization Volume 896 on EtoBox?

Advanced Technologies for Protein Complex Production and Characterization Volume 896 by Vega, M. Cristina is a scholarly article available to read on EtoBox.

What is Advanced Technologies for Protein Complex Production and Characterization Volume 896 about?

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli , yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa ( Leishmania terantolae and Dictyostelium discoideum ), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nu

Author

Vega, M. Cristina

Publisher

Springer International Publishing : Springer e-books : Imprint : Springer : Springer e-books

Published

2016

Language

EN

ISBN

9783319272160