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IgE–Mediated Reactions to Autoantigens in Allergic Diseases by Ulrich Appenzeller; Christina Meyer; Günter Menz; Kurt Blaser; Reto Crameri is a Medicine article available to read on EtoBox.

What is IgE–Mediated Reactions to Autoantigens in Allergic Diseases about?

**Background:** There is increasing evidence that IgE–mediated reactivity against autoantigens could play a role in the pathology of severe chronic atopic diseases. **Methods:** Human mitochondrial managenese superoxide dismutase (MnSOD), acidic ribosomal P~2~ protein and cyclophilins were cloned by PCR, expressed as inclusion body proteins in __Escherichia coli__, purified by Ni^2+^–chelate affinity chromatography, refolded in vitro and used for further experiments. **Results:** Human MnSOD, P~2~ ribosomal protein and cyclophilins respectively, show sequence identity and similarity of >50% and 70% to the corresponding allergens of __Aspergillus fumigatus__. The human proteins were able to bind IgE from sera of individuals sensitized to the mould proteins and to elicit strong skin reactions of the immediate type in these individuals. **Conclusions:** These observations provide evidence for autoreactivity to human proteins in allergic persons chronically sensitized to environmental allergens from __A. fumigatus__ which share a high degree of sequence homology to the corresponding human proteins.

Who reads IgE–Mediated Reactions to Autoantigens in Allergic Diseases?

Author

Ulrich Appenzeller; Christina Meyer; Günter Menz; Kurt Blaser; Reto Crameri

Publisher

S. Karger AG

Published

1999

Language

EN

Field

Medicine (Health Sciences)