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HyperTRIBE mapping of the RNA m°A demethylase ALKBH9 binding sites 2 in bamboo reveals its role in plant defense by Huihui Wang't, Huiyuan Wang'f, Yue Jia, Tuhe Li', Siyu láng?, Yandong Jin, Zaofa is a nonfiction available to read on EtoBox.
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AbstractRNA demethylation plays an important role in diverse biological processes. Intriguingly, RNA demethylation has not been reported in bamboo, which is known for its rapid growth.PheALKBH9, an mA demethylase in bamboo, was stably transfomed into rice and increased its susceptibility to rice blast disease. Heterologous expression of PheALKBH9 reduced the overall m'A modification levels in rice. Using HyperTRIBE (Targets of RNA-binding protcins Identified By Editing), we identified evolutionarily conserved PheALKBH9 target RNAs in both rice and Moso bamboo. Overexpression of PheALKBH9 led to higher protein expression and shorter poly(A) tails. Notably, PheALKBH9 directly bound to CCR4-associated factorl (CAFIG) and © The Author(s) 2025. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, pleasecontact reprints @oup.comfor reprints and translation rights forreprints. All other permissions can be obtained through our RightsLinkservicevia the Permissions link on the article page on our site-for further information [email protected]
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- Author
- Huihui Wang't, Huiyuan Wang'f, Yue Jia, Tuhe Li', Siyu láng?, Yandong Jin, Zaofa
- Published
- 2025
- Language
- EN
- Category
- nonfiction
- Subjects
- Science, Genetics, Stem
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