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Acquisition of iron from human transferrin by Porphyromonas gingivalis: a role for Arg- and Lys-gingipain activities by V. Brochu; D. Grenier; K. Nakayama; D. Mayrand is a Dentistry article available to read on EtoBox.

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__Porphyromonas gingivalis__, a key causative agent of adult periodontitis, is known to produce a variety of virulence factors including proteases. The aim of this study was to evaluate the participation of Arg‐ and Lys‐gingipain activities of __P. gingivalis__ in the acquisition of iron from human transferrin and its subsequent utilization in growth. Iron‐saturated transferrin was found to support the long‐term growth of __P. gingivalis__. Our results indicated that __P. gingivalis__ does not produce siderophore and does not possess ferric reductase and transferrin‐binding activities. Incubating transferrin with __P. gingivalis__ resulted in degradation of the protein, a step that may be critical for the acquisition of iron from transferrin. Spontaneous and site‐directed mutants of __P. gingivalis__ deficient in one or several proteases were used to demonstrate the key role of specific enzymes in degradation of transferrin and subsequent utilization for growth. The lack of both Arg‐ and Lys‐gingipain activities (mutants M1 and KDP128) was associated with an absence of degradation of transferrin and the incapacity of bacteria to grow in the presence of transferrin as the sole sourc

Who reads Acquisition of iron from human transferrin by Porphyromonas gingivalis: a role for Arg- and Lys-gingipain activities?

Author

V. Brochu; D. Grenier; K. Nakayama; D. Mayrand

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0902-0055)

Published

2001

Language

EN

Field

Dentistry (Health Sciences)