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Heterogeneity of Mesenchymal and Pluripotent Stem Cell Populations Grown on Nanogrooves and Nanopillars by Peng-Yuan Wang; Sheryl Ding; Huseyin Sumer; Raymond Ching-Bong Wong; Peter Kingshott is a Materials Science article available to read on EtoBox.

What is Heterogeneity of Mesenchymal and Pluripotent Stem Cell Populations Grown on Nanogrooves and Nanopillars about?

Surface nanotopographies are an important way of mimicking the stem cell niche on biomaterial surfaces. Previous studies have focused on the differentiation of stem cells into a defined lineage using nanotopographies, but they have rarely considered the homogeneity of cell populations produced. We examined the impact of two types of substrates (i.e. nanogrooves and nanopillars made by soft lithography) on the surface-induced differentiation of human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) and mouse embryonic stem cells (mESCs) without the use of additional chemical induction medium components. Cell morphology and proliferation were analysed at day 1 and day 3. Gene expression was analysed at day 14 for hAM-MSCs and at day 7 for mESC-derived embryoid bodies (mEBs) using quantitative real-time polymerase chain reaction (qPCR). The substrates with nanogrooves had a noticeable effect on cell alignment in a depth dependent manner with both cell types showing strong alignment along the deep grooves. On the other hand, the nanopillar substrates showed inhibition of cell spreading for both cell types. The nanogrooves showed inhibition of hAM-MSC growth but enhanced mEB

Who reads Heterogeneity of Mesenchymal and Pluripotent Stem Cell Populations Grown on Nanogrooves and Nanopillars?

Author

Peng-Yuan Wang; Sheryl Ding; Huseyin Sumer; Raymond Ching-Bong Wong; Peter Kingshott

Publisher

Royal Society of Chemistry (RSC)

Published

2017

Language

EN

Field

Materials Science (Physical Sciences)