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The Application of Papain, Ficin and Clostripain in Kinetically Controlled Peptide Synthesis in Frozen Aqueous Solutions by Dr Marion Hänsler; Grit Ullmann; Hans-Dieter Jakubke is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The Application of Papain, Ficin and Clostripain in Kinetically Controlled Peptide Synthesis in Frozen Aqueous Solutions about?

## Abstract The capability of the cysteine proteases ficin, papain and clostripain to form peptide bonds in frozen aqueous solutions was investigated. Freezing the reaction mixture resulted in increased peptide yields in kinetically controlled coupling of Bz–Arg–OEt with various amino acid amides and dipeptides. Under these conditions, peptide yields increased up to 70% depending on the enzyme and the amino component used. Enzyme‐catalysed peptide syntheses were carried out under optimized reaction conditions (temperature, amino component concentration and pH before freezing) using the condensation of Bz–Arg–OEt and H–Leu–NH~2~ as a model reaction.

Who reads The Application of Papain, Ficin and Clostripain in Kinetically Controlled Peptide Synthesis in Frozen Aqueous Solutions?

Author

Dr Marion Hänsler; Grit Ullmann; Hans-Dieter Jakubke

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-2617)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)