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Can I read Epigenetically-regulated miR-30a/c-5p directly target TWF1 and hamper ccRCC cell aggressiveness on EtoBox?
Epigenetically-regulated miR-30a/c-5p directly target TWF1 and hamper ccRCC cell aggressiveness by GONÇALO OUTEIRO-PINHO; DANIELA BARROS-SILVA; FILIPA MOREIRA-SILVA; JOÃO LOBO; ISA CARNEIRO; ANTÓNIO MORAIS; EDUARDA P. MARTINS; CÉLINE S. GONÇALVES; BRUNO M. COSTA; MARGARETA P. CORREIA; RUI HENRIQUE; CARMEN JERÓNIMO is a Economics, Econometrics and Finance article available to read on EtoBox.
What is Epigenetically-regulated miR-30a/c-5p directly target TWF1 and hamper ccRCC cell aggressiveness about?
Clear cell renal cell carcinoma (ccRCC) is highly prone to metastasize and displays an extremely low 5-year survival rate. Not only miRNAs (miRs) are key gene expression regulators but can also be epigenetically modified. Abnormal miR expression has been linked with epithelial-mesenchymal transition (EMT)-driven ccRCC progression. MiR-30a/c-5p were found downregulated in ccRCC and associated with aggressiveness. Herein, we sought to unravel miR-30a/c-5p mechanistic role in ccRCC. RNA sequencing and genome-wide methylome data of ccRCC and normal tissue samples from The Cancer Genome Atlas database were integrated to identify candidate miRs cytosine-phosphate-guanine (CpG) loci deregulated in ccRCC. TargetScan was searched to identify miR putative targets. MiR-30a/c-5p expression and promoter methylation was evaluated in vitro, by PCR. Western blot, functional and luciferase assays were performed after cell transfection with either pre-miR, antimiR, or siRNA against twinfilin-1 (TWF1). Immunohistochemistry (IHC) was performed in ccRCC tissues. We found miR-30c-5p downregulation and aberrant promoter methylation in ccRCC tissues. In vitro studies revealed concomitant miR-30a/ c-5p dow
Who reads Epigenetically-regulated miR-30a/c-5p directly target TWF1 and hamper ccRCC cell aggressiveness?
It is typically read by researchers, students, and practitioners in Economics, Econometrics and Finance.
- Author
- GONÇALO OUTEIRO-PINHO; DANIELA BARROS-SILVA; FILIPA MOREIRA-SILVA; JOÃO LOBO; ISA CARNEIRO; ANTÓNIO MORAIS; EDUARDA P. MARTINS; CÉLINE S. GONÇALVES; BRUNO M. COSTA; MARGARETA P. CORREIA; RUI HENRIQUE; CARMEN JERÓNIMO
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Economics, Econometrics and Finance (Physical Sciences)