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Cross-talk between ammonium transporters in yeast and interference by the soybean SAT1 protein by Anne-Marie Marini; Jean-Yves Springael; Wolf B. Frommer; Bruno André is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cross-talk between ammonium transporters in yeast and interference by the soybean SAT1 protein about?
Ammonium uptake in the yeast __Saccharomyces cerevisiae__ involves three membrane transporters (Mep1, ‐2 and ‐3) belonging to an evolutionarily conserved protein family that also includes the rhesus (Rh) blood group polypeptides of erythrocytes. We show here that, in the 26972c mutant defective in NH~4~^+^ transport, the Mep1 protein carrying an amino acid substitution in its cytoplasmic C‐terminus __trans__‐inhibits the closely related Mep3 protein. The same mutation introduced into Mep3 leads to loss of transport activity and this inactive form also __trans__‐inhibits native Mep3. Inhibition of Mep3 is post‐translational and can be overcome by overexpression. These results are consistent with a direct interaction between Mep proteins, as is the case for the Rh polypeptides. The soybean __GmSAT1__ gene, recently cloned for its ability to complement the NH~4~^+^ transport defect of strain 26972c, has been described as an NH~4~^+^ channel protein involved in the transfer of fixed nitrogen from the bacteroid to the host plant. We show here that GmSAT1 contains a sequence homologous to the DNA‐binding domain of basic helix–loop–helix (bHLH) transcription factors. We also show that GmS
Who reads Cross-talk between ammonium transporters in yeast and interference by the soybean SAT1 protein?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anne-Marie Marini; Jean-Yves Springael; Wolf B. Frommer; Bruno André
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)