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The UCHL5 inhibitor b-AP15 overcomes cisplatin resistance via suppression of cancer stemness in urothelial carcinoma 1 Introduction 1 Results 3 UCHL5 and USP14 are increased in bladder UC 3 b-AP15 increases the accumulation of polyubiquitinated proteins and induces ER stress 3 b-AP15 decreases cell viability and induces apoptosis in bladder UC 3 b-AP15 suppresses cancer stemness by inhibiting the β-catenin and c-myc signaling pathways 3 b-AP15 and cisplatin exert synergistic effects in bladder UC by suppressing β-catenin and myc expression 3 b-AP15 reduces tumor growth in mouse xenograft models 3 Discussion 5 Materials and methods 5 Reagents and antibodies 5 IHC 5 Cell culture and the chemo-resistant strain 6 Cell viability assay 8 Combination index 8 Immunoblotting 10 Quantitative real-time PCR 10 Flow cytometry 10 Tumorsphere cultivation 10 RNA-seq analysis 10 UC xenograft model 10 Statistical analysis 10 Data availability statement 11 Supplemental information 11 Acknowledgments 11 Author contributions 11 Declaration of interests 11 References 11

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Language

UND

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nonfiction