Can I read Adenovirus-mediated Gene Transfer of Wild-type P53 Results in Melanoma Cell Apoptosis in Vitro and in Vivo on EtoBox?

Adenovirus-mediated Gene Transfer of Wild-type P53 Results in Melanoma Cell Apoptosis in Vitro and in Vivo by Corrado Cirielli; Teresa Riccioni; Chunlin Yang; Roberto Pili; Torsten Gloe; Joan Chang; Kyosuke Inyaku; Antonino Passaniti; Maurizio C. Capogrossi is a Medicine article available to read on EtoBox.

What is Adenovirus-mediated Gene Transfer of Wild-type P53 Results in Melanoma Cell Apoptosis in Vitro and in Vivo about?

## Abstract Gene transfer techniques may provide efficient treatment for a variety of malignant neoplasms. A replication‐deficient adeno‐virus (Ad) vector which carries the cDNA for wild‐type p53 (AdCMV.p53) was tested for its __in vitro__ and __in vivo__ effects on the growth of murine melanoma cell line B16‐G3.26 and human melanoma cell line SK‐MEL‐24. The growth of B16‐G3.26 cells infected with AdCMV.p53 was inhibited when compared to the uninfected cells or cells infected with the control vector AdCMV.NLSβgal. Similarly, the growth of SK‐MEL‐24 cells infected with AdCMV.p53 was also below that of AdCMV.NLSβgal‐infected and uninfected controls. DNA laddering using agarose gel electrophoresis and __in situ__ labeling of DNA fragmentation (TUNEL) showed that AdCMV.p53‐infected murine and human melanoma cells underwent apoptosis. Nude mice injected s.c. either with B16‐G3.26 cells or with SK‐MEL‐24 cells developed localized tumors. These tumors were subsequently infiltrated with either AdCMV.p53, AdCMV.NLSβgal or saline alone. One week after infection, B16‐G3.26 tumors exposed to AdCMV.p53 were 2.5 times smaller than control tumors and exhibited DNA fragmentation. A similar growth‐

Who reads Adenovirus-mediated Gene Transfer of Wild-type P53 Results in Melanoma Cell Apoptosis in Vitro and in Vivo?

Author

Corrado Cirielli; Teresa Riccioni; Chunlin Yang; Roberto Pili; Torsten Gloe; Joan Chang; Kyosuke Inyaku; Antonino Passaniti; Maurizio C. Capogrossi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1995

Language

EN

Field

Medicine (Health Sciences)