Can I read Application of the flow field flow fractionation (FFFF) to the characterization of aquatic humic colloids: evaluation and optimization of the method on EtoBox?

Application of the flow field flow fractionation (FFFF) to the characterization of aquatic humic colloids: evaluation and optimization of the method by Ngo Manh Thang; H Geckeis; J.I Kim; H.P Beck is a Materials Science article available to read on EtoBox.

What is Application of the flow field flow fractionation (FFFF) to the characterization of aquatic humic colloids: evaluation and optimization of the method about?

The flow field flow fractionation (FFFF) is applied to determine the aquatic humic colloid size distribution. In order to determine average molecular weights of various aquatic humic acids, a calibration is carried out by using sulfonated polystyrene (PSS) reference colloids. Some interference is found to be inherent to the FFFF-method. Considerable sorption of humic substances to equipment components is observed depending on the ionic strength and pH of given carrier medium, the kind of membrane covering the accumulation wall, and the cross-flow rate. The present study shows that the sample recovery can be optimized by selecting a carrier of low ionic strength and high pH (5×10 -3 mol l -1 Tris-buffer; pH =9.1) and using a regenerated cellulose membrane (cutoff: 5 kDa related to globular proteins). Under these low ionic strength conditions, the molecular weight calibration by using sulfonated polystyrene standards (PSS) is affected by 'overloading' effects originating from charge repulsive interaction of particles with one another and also with the accumulation wall. In general, the elution time of PSS decreases with increasing concentration. A correction for the 'overloading' eff

Who reads Application of the flow field flow fractionation (FFFF) to the characterization of aquatic humic colloids: evaluation and optimization of the method?

Author

Ngo Manh Thang; H Geckeis; J.I Kim; H.P Beck

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7757)

Published

2001

Language

EN

Field

Materials Science (Physical Sciences)