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Meta-transcriptomic analysis reveals an isolate of aphid lethal paralysis virus from Wisteria sinensis in Iran 1 1 Introduction 1 2 Material and methods 2 2.1 RNA isolation, cDNA library preparation, and RNA sequencing 2 2.2 Recombination analysis 2 2.3 Phylogenetic and sequence analysis 2 2.4 Population evolution 2 3 Results 3 3.1 Genome structure 3 3.2 Analysis of ALPV sequences 3 3.3 Recombination analyses 3 3.4 Phylogenetic analyses 4 3.5 Population genetic structure of the isolates 7 3.6 Test of neutrality 7 3.7 Genetic differentiation of phylogroups and geographical populations in the ORF2 8 4 Discussion 8 CRediT authorship contribution statement 9 Declaration of Competing Interest 9 Ethical approval 9 Supplementary materials 9 References 9
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