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Protein Labeling and Crosslinking by Covalent Aptamers by Yaniv Tivon; Gianna Falcone; Alexander Deiters is a Chemistry article available to read on EtoBox.

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## Abstract We developed a new approach to selectively modify native proteins in their biological environment using electrophilic covalent aptamers. These aptamers are generated through introduction of a proximity‐driven electrophile at specific nucleotide sites. Using thrombin as a proof‐of‐concept, we demonstrate that covalent aptamers can selectively transfer a variety of functional handles and/or irreversibly crosslink to the target protein. This approach offers broad programmability and high target specificity. Furthermore, it addresses issues common to aptamers such as instability towards endogenous nucleases and residence times during target engagement. Covalent aptamers are new tools that enable specific protein modification and sensitive protein detection. Moreover, they provide prolonged, nuclease‐resistant enzyme inhibition.

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Author

Yaniv Tivon; Gianna Falcone; Alexander Deiters

Publisher

Wiley

Published

2021

Language

EN

Field

Chemistry (Physical Sciences)