Can I read 484 Melatonin induces melanoma cell death and synergizes with BRAF/MEK inhibitors by altering cell bioenergetics on EtoBox?

484 Melatonin induces melanoma cell death and synergizes with BRAF/MEK inhibitors by altering cell bioenergetics by J. Möller; A. Wolnicka-Glubisz; F. Neesen; C.G. Becker; V. Raker; R. Dreier; M.K. Tulic; K. Steinbrink; M.B. öhm; K. Kleszczynski is a Medicine article available to read on EtoBox.

What is 484 Melatonin induces melanoma cell death and synergizes with BRAF/MEK inhibitors by altering cell bioenergetics about?

̈nster, Mu ̈nster, Germany and 4 Centre Me ́diterrane ́en de Me ́decine Mole ́culaire (C3M), Universite ́Co ˆte d'Azur, Nice, France Melanoma is a leading cause of cancer deaths worldwide. Although targeted therapy and immunotherapy have improved the outcome of patients with metastatic disease, unwanted side effects are a problem. Herein, by using human melanoma cell lines in vitro, we explore that melatonin enhances anti-tumor activity of commonly used BRAF/MEK inhibitors, i.e. vemurafenib (VF) and cobimetinib (CB), respectively. Our results have demonstrated that compared with VF/CB alone, melatonin significantly reduced proliferation read-outs (colony assay, drop assay) and induced of apoptosis (cleaved Casp-3, PARP) in melanoma cells. Concurrently, VF/CB+melatonin decreased melanoma invasiveness-related protein (E-cadherin), inducible nitric oxide synthase (iNOS), epithelial cell adhesion molecule (EpCAM), and proliferating cell nuclear antigen (PCNA); important players in melanoma tumorgenesis, tumor growth, invasion and metastasis. In addition, we also found that the combined treatment caused significant mechanistic changes in cellular bioenergetics by (i) uncoupling of

Who reads 484 Melatonin induces melanoma cell death and synergizes with BRAF/MEK inhibitors by altering cell bioenergetics?

Author

J. Möller; A. Wolnicka-Glubisz; F. Neesen; C.G. Becker; V. Raker; R. Dreier; M.K. Tulic; K. Steinbrink; M.B. öhm; K. Kleszczynski

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Medicine (Health Sciences)