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KPC-2-producing Klebsiella pneumoniae ST147 in a neonatal unit: Clonal isolates with differences in colistin susceptibility attributed to AcrAB-TolC pump by Sharmi Naha; Kirsty Sands; Subhankar Mukherjee; Chayan Roy; Moidu Jameela Rameez; Bijan Saha; Shanta Dutta; Timothy R. Walsh; Sulagna Basu is a Medicine article available to read on EtoBox.

What is KPC-2-producing Klebsiella pneumoniae ST147 in a neonatal unit: Clonal isolates with differences in colistin susceptibility attributed to AcrAB-TolC pump about?

This study characterizes four KPC-2-producing Klebsiella pneumoniae isolates from neonates belonging to a single sequence type 147 (ST147) in relation to carbapenem resistance and explores probable mechanisms of differential colistin resistance among the clonal cluster.Whole genome sequencing (WGS) revealed that the isolates were nearly 100% identical and harbored resistance genes ( bla KPC-2 , OXA-9 , CTX-M-15 , SHV-11 , OXA-1 , oqxA, oqxB, qnrB1, fosA, sul1, aacA4, aac(6 ) Ibcr, aac(6 )Ib ) , and several virulence genes . bla KPC-2 was the only carbapenem-resistant gene found, bracketed between IS Kpn7 and IS Kpn6 of Tn 4401 b on a non-conjugative IncFII plasmid. Remarkably, one of the clonal isolates was resistant to colistin, the mechanistic basis of which was not apparent from comparative genomics. The transmissible colistin resistance gene, mcr, was absent. Efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) rendered a 32-fold decrease in the minimum inhibitory concentration (MIC) of colistin in the resistant isolate only. acrB, tolC, ramA, and soxS genes of the AcrAB-TolC pump system overexpressed exclusively in the colistin-resistant isolate, although th

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Author

Sharmi Naha; Kirsty Sands; Subhankar Mukherjee; Chayan Roy; Moidu Jameela Rameez; Bijan Saha; Shanta Dutta; Timothy R. Walsh; Sulagna Basu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0924-8579)

Published

2020

Language

EN

Field

Medicine (Health Sciences)