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The Effect of Neutral Salt Anions on the Oxidative Deamination Activity of Plant Glutamate Dehydrogenase by Edwin Pahlich; Balazs Gelleri; Rainer Kindt is a Agricultural and Biological Sciences article available to read on EtoBox.

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Increasing concentrations of anions of the Hofmeister series decrease the activity of highly purified glutamate dehydrogenase (EC 1.4.1.2.) from Pi- sum s a t i v u m L. The extent of the inactivation, as estimated by the ion concentration which causes a 50% t r a n s f o r m a t i o n of the native form to the low activity form of the enzyme ( a p p r o x i m a t e l y " h a l fm a x i m a l activity "), follows the ranking C1 < F < B r -< N O 3 < S C N -. Sulfate has a slightly activating effect. A t salt concentrations higher than 1 M (with S C N -higher than 200mM), the activity decreases to a value from 3-6 % of the initial activity and remains then stable over a wide range of higher anion concentrations. F r o m kinetic investigations it is seen that the treatment of the enzyme with anions decreases the affinity for the cosubstrate N A D + and the substrate L -g l u t a m a t e (KM-Values increased) and also increases the dissociation constant for N A D § . The salt induced inactivation is reversible by dilution. F r o m a mathematical treatment of the kinetic d a t a of the inactivation, it is seen that increasing concentrations of the anions exert cooperative effects on the

Who reads The Effect of Neutral Salt Anions on the Oxidative Deamination Activity of Plant Glutamate Dehydrogenase?

Author

Edwin Pahlich; Balazs Gelleri; Rainer Kindt

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1978

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)