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Tissue-nonspecific Alkaline Phosphatase Is Activated in Enterocytes by Oxidative Stress via Changes in Glycosylation by Rocío López-Posadas; Raquel González; Isabel Ballester; Patricia Martínez-Moya; Isabel Romero-Calvo; María Dolores Suárez; Antonio Zarzuelo; Olga Martínez-Augustin; Fermín Sánchez de Medina is a Medicine article available to read on EtoBox.
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## Background: Intestinal inflammation produces an induction of alkaline phosphatase (ap) activity that is attributable in part to augmented expression, accompanied by a change in isoform, in epithelial cells. ## Methods: This study focuses on induction of ap in intestinal epithelial cells in vitro. ## Results: Treatment with the oxidants h2o2, monochloramine, or tbutooh increases ap activity in vitro in caco-2, ht29, and iec18 cells. we selected iec18 cells for further testing. basal ap activity in iec18 cells is of the tissue-nonspecific (bone-liver-kidney) type, as indicated by northern and western blot analysis. oxidative stress augments ap activity and the sensitivity of the enzyme to levamisole, homoarginine, and heat in iec18 cells. increased immunoreactivity to tissue-nonspecific ap antibodies suggests an isoform shift from liver to either kidney or bone type. this effect occurs without changes at the mrna level and is sensitive to tunicamycin, an inhibitor of n-glycosylation, and neuraminidase digestion. saponin and deoxycholate produce similar effects to oxidants. butyrate but not proinflammatory cytokines or lps can induce a similar effect but without toxicity. the ap in
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- Author
- Rocío López-Posadas; Raquel González; Isabel Ballester; Patricia Martínez-Moya; Isabel Romero-Calvo; María Dolores Suárez; Antonio Zarzuelo; Olga Martínez-Augustin; Fermín Sánchez de Medina
- Publisher
- John Wiley and Sons; Oxford University Press; Oxford University Press (OUP) (ISSN 1078-0998)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)