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Long non-coding (lnc)RNA profiling and the role of a key regulator lnc-PNRC2-1 in the transforming growth factor-**β**1-induced epithelial–mesenchymal transition of CNE1 nasopharyngeal carcinoma cells by Jie Yang; Enzi Feng; Yanxin Ren; Shun Qiu; Liufang Zhao; Xiaojiang Li is a Medicine article available to read on EtoBox.

What is Long non-coding (lnc)RNA profiling and the role of a key regulator lnc-PNRC2-1 in the transforming growth factor-**β**1-induced epithelial–mesenchymal transition of CNE1 nasopharyngeal carcinoma cells about?

## Objectives To identify key long non-coding (lnc)RNAs responsible for the epithelial–mesenchymal transition (EMT) of CNE1 nasopharyngeal carcinoma cells and to investigate possible regulatory mechanisms in EMT. ## Methods CNE1 cells were divided into transforming growth factor (TGF)-β1-induced EMT and control groups. The mRNA and protein expression of EMT markers was determined by real-time quantitative PCR and western blotting. Differentially expressed genes (DEGs) between the two groups were identified by RNA sequencing analysis, and DEG functions were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses. EMT marker expression was re-evaluated by western blotting after knockdown of a selected lncRNA. ## Results TGF-β1-induced EMT was characterized by decreased E-cadherin and increased vimentin, N-cadherin, and Twist expression at both mRNA and protein levels. Sixty lncRNA genes were clustered in a heatmap, and mRNA expression of 14 dysregulated lncRNAs was consistent with RNA sequencing. Knockdown of lnc-PNRC2-1 increased expression of its antisense gene __MYOM3__ and reduced expression of EMT markers, resembling treatment with the TGF-β1 receptor inhi

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Author

Jie Yang; Enzi Feng; Yanxin Ren; Shun Qiu; Liufang Zhao; Xiaojiang Li

Publisher

SAGE Publications

Published

2021

Language

EN

Field

Medicine (Health Sciences)