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Mitogenic G~i~ protein‐MAP kinase signaling cascade in MC3T3‐E1 osteogenic cells: Activation by C‐terminal pentapeptide of osteogenic growth peptide [OGP(10–14)] and attenuation of activation by cAMP by Nidal Gabarin; Hanna Gavish; Andras Muhlrad; Yu‐Chen Chen; Malka Namdar‐Attar; Robert A. Nissenson; Michael Chorev; Itai Bab is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In osteogenic and other cells the mitogen‐activated protein (MAP) kinases have a key role in regulating proliferation and differentiated functions. The osteogenic growth peptide (OGP) is a 14 mer mitogen of osteogenic and fibroblastic cells that regulates bone turnover, fracture healing, and hematopoiesis, including the engraftment of bone marrow transplants. It is present in the serum and extracellular fluid either free or complexed to OGP‐binding proteins (OGPBPs). The free immunoreactive OGP consists of the full length peptide and its C‐terminal pentapeptide OGP(10–14). In the present study, designed to probe the signaling pathways triggered by OGP, we demonstrate in osteogenic MC3T3 E1 cells that mitogenic doses of OGP(10–14), but not OGP, enhance MAP kinase activity in a time‐dependent manner. The OGP(10–14)‐induced stimulation of both MAP kinase activity and DNA synthesis were abrogated by pertusis toxin, a G~i~ protein inhibitor. These data offer direct evidence for the occurrence in osteogenic cells of a peptide‐activated, mitogenic Gi protein‐MAP kinase‐signaling cascade. Forskolin and dBu~2~‐cAMP abrogated the OGP(10–14)‐stimulated proliferation, but induced o

Who reads Mitogenic G~i~ protein‐MAP kinase signaling cascade in MC3T3‐E1 osteogenic cells: Activation by C‐terminal pentapeptide of osteogenic growth peptide [OGP(10–14)] and attenuation of activation by cAMP?

Author

Nidal Gabarin; Hanna Gavish; Andras Muhlrad; Yu‐Chen Chen; Malka Namdar‐Attar; Robert A. Nissenson; Michael Chorev; Itai Bab

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)